Prof. D.K. Das
Prof. D.K. Das
T.M.Bhagalpur University, India
Title: Expression of a bacterial chitinase (ChiB) gene enhances resistance against E. polygoni induced powdery mildew disease in the transgenic Black gram (Vigna mungo L.) (cv. T9)
To enhance the antifungal response of blackgram (Vigna mungo L.), transgenic plants were generated by transferring bacterial chitinase gene with a CaMV 35S promoter. The chopped multiple shoot cells developed on the cotyledonary node were transformed by Particle gun method. The calli were raised on the Murashige and Skoog (MS) modified media supplemented with 50mgl-1 kanamycin. The transformation efficiency was 13%. The resultant shoot buds were selected and the antibiotic resistant transgenic plantlets were regenerated. The development of the transgenic plants from the shoot buds took about four to six months. The transgenic status was confirmed by PCR, RT-PCR, Southern and western blot analyses. The transgenic plants exhibited higher chitinase activity than the non-transformed plants. The chitinase activity was examined using the native polyacrylamide in-gel assay. The transgenic plants showed enhanced resistance as evidenced by the delayed onset of the disease and smaller lesions following an in vitro inoculation of the powdery mildew pathogen (E. polygoni DC). The transgenic plants adapted well to the greenhouse and did not show any phenotypic alterations.
Key words Vigna mungo L., Bacterial chitinase, transgenic plants, Fungal resistance, E. polygoni
I, Dr. D.K.Das, Senior Assistant Professor, G.B.College, Naugachia and also deputed in P.G.Deptt. of Biotechnology, and also teaches in University Department of Botany,T.M.Bhagalpur University, Bhagalpur, India. I received both B.Sc. and M.Sc. degrees in Botany from T.M. Bhagalpur University, Bhagalpur and did Ph.D. degree in Plant Physiology and Biochemistry from the same University and did Post doctoral researches in JNU, New Delhi on Plant Tissue culture and Molecular Biology, in ICGEB, New Delhi on cotton transformation and in University of Washington, Seattle, USA on Characterization of TnPhoA mutants of Agrobacterium tumefaciens. I have published many research papers in international journals of repute. I have participated and presented research papers in both national as well as in International symposia. I received National Associateship award in 1994 and Overseas Associateship award in 1999 from Deptt. Of Biotechnology, Govt. of India, INSA visiting fellowship award in 1996 and 1997 from INSA Govt. of India, UGC sponsored visiting fellowship in 2010. I also received Shiksha Rattan Puruskar at India International Centre, New Delhi, UGC sponsored Visiting Fellowship (2010) for seven days i.e. 02 to 08th March at SGB Amravati University, Amravati, Maharashtra,Travel grant was awarded from UGC, New Delhi to attend International Conference, BIT’S 3rd Annual World Congress on Microbes (WCM-2013) 2013, from July 30th –August 1st, Wuhan, China. I have already transformed grape, cotton, blackgram and litchi. My main focus on plant transformation and expressing genes in plants for fungal tolerance, viral tolerance, insect tolerance and salt tolerance.