| Biography | |
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 Prof. Priti Mathur Amity University Uttar Pradesh, India |
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| Title: An Electron Microscopic Study to analyse the effect of Dinoxin B With an olide against Staphylococcus aureus | |
| Abstract: We have isolated and characterize of antibacterial compound Dinoxin B Withanolide from ethanolic leaf extract of Datura inoxia(alreadyreported). The compound showed significant inhibition (bactericidal)on both standard and clinical strains of Staphylococcus aureus. To understand mechanisms of action of this compound and to further confirm its antibacterial potential, present study was conducted to determine morphological and ultra-structural deformities in the Staphylococcus aureus when treated with Dinoxin B Withanolide.We have studied structural changes of control and treated bacteria by scanning electron microscope (SEM) and transmission electron microscope (TEM). Electron Microscopy (EM) is a very important technique for obtaining high resolution images of microscopic organisms, cells and tissues. The main abnormalities observed via SEM were the alteration in morphology of the bacterial cells. Untreated cells were found spherical and regular, while after treatment cells became damaged and irregular. In TEM micrograph, the control cell wall and membrane were found intact, while in treated bacteria, cell wall was found broken, resulting in cell death.It was found that Dinoxin B withanolide destroy bacterial cell wall and membrane, enter into cytoplasm. This causes complete degradation of DNA and protein, which hampered bacterial growth and finally resulted in the bacterial death. These results are in accordance with our bioassay results and confirm the bactericidal action of the Dinoxin B with an olide, suggesting its potential as a candidate drug. Natural compounds have much more complex chemistry than synthetic compounds. Due to complex structure of phytocompoundsbacteria cannot quickly counter their effects and cannot develop immunity to them. Instead of developing synthetic antibacterial therapy, effort should be on research on natural antibiotics. It would not only take us closer to nature, but several new healthier ways of living a disease free life will be discovered. To the best of our knowledge, we are the first to identify, Dinoxin B with an olide as an antibacterial compound from D. inoxiα.To further confirm its therapeutic applicability, studies on animal models required followed by pharmaceutical formulation and clinical trials for the welfare of mankind. KEY WORDS:Datura innoxia,Staphylococcus aureus, Dinoxin B withanolide,Agar Gel Electrophoresis,polyacrylamide gel electrophoresis, scanning electron microscopy, transmission electron microscopy | |
| Biography: Dr Priti Mathur is working in Amity Institute of Biotechnology, Amity University Uttar Pradesh, and Lucknow as Assistant professor. Dr Priti Mathur has provedfirst time in world that antibiotics like compounds can be isolated from plant source. This is evident by her recent research papers published in national and international journal and patent“herbal antibiotic formulation” submitted to patent office, Government of India.Dr Priti Mathur is working on “Isolation and characterization of antibacterial compounds from wildly growing plants” with her students withcollaboration with Ram Manohar Lohia Institute of Medical Sciences and Central drug research institute, Lucknow. Dr Mathur completed herPost graduation Patna University, Bihar India. Dr Priti Mathur has done PhD from CSIR organisation- Centre Institute of Medicinal and Aromatic Plants and Post doctorate from ICAR organization -Indian Institute of Sugarcane research. She has filed two patents and published many research papers in reputed national/ international journal and presented her work in various national/international conferences.She has been awarded by fellowships and award for her research work. | |
