Biography
Dr. SANJAY SINGH NEGI
Dr. SANJAY SINGH NEGI
Department of Microbiology, All India Institute of Medical Sciences(AIIMS), Raipur, Chhattisgarh, India
Title: Comparative evaluation of Gene Xpert with commercial available real time PCR kit (Gene Proof) for detection of extra-pulmonary tuberculosis: A preliminary data
Abstract: 
Comparative evaluation of Gene Xpert with commercial available real time PCR kit (Gene Proof) for detection of extra-pulmonary tuberculosis: A preliminary data Author: Sanjay Singh Negi*, Sachin Chandrakar, Anudita Bhargava. Abstract: The emergence of the more worrisome forms of multidrug resistant, extensive drug resistant and total drug resistant tuberculosis (MDR, XDR & TDR-TB) has posed a serious threat to its management and requires a rapid detection of Mycobacterium tuberculosis for effective early disease management. Gene Xpert, has although been approved by WHO for rapid diagnosis of pulmonary tuberculosis, is found to have shown a low sensitivity in detecting various forms of extra-pulmonary TB. In the present study a total of 50 samples of clinically suspected cases of tuberculosis of multi varied nature viz., endometrial biopsy(20), menstrual blood (n=02), synovial fluid(n=12), pleural fluid(n=06), peritoneal fluid(n=01), Ascitic fluid(n=02) skin abscess(n=01), skin biopsy(n=01), lymph node aspirate (n=01), pus(n=02) and urine(n=01) from equal number of patients were included. All the samples were found negative in microscopy. All the samples were simultaneously tested by both Gene Xpert targeting rpo B gene and real time PCR based commercial kit (Gene proof, Czech Republic) targeting IS6110 multicopy insertion sequence. Result: A total of 6 samples tested positive for Mycobacterium DNA by Real time PCR kit whereas Gene Xpert detected Mycobacterium DNA in 2 of these positive samples. Conclusion: The preliminary data from this on-going study points at higher detection rate of paucibacillary extrapulmonary TB by RT-PCR commercial kit in comparison to GeneXpert & microscopy. This is however, just a preliminary data and a more comprehensive interpretation can be deduced after analyzing at least 200 extra-pulmonary specimen from clinically suspected cases and comparing the results with that from culture positivity and/or response to Anti-tubercular treatment. • Presenting author Dr. Sanjay Singh Negi Assistant Professor
Biography: 
Dr. Sanjay Singh Negi, Ph.D(Microbiology), is presently working as an Associate Professor in the Department of Microbiology in All India Institute of Medical Sciences(AIIMS), Raipur, Chhattisgarh.
He has been working in the field of Microbiology since 2002 and has worked in variousprestigious government medical and research institute namely Central Research Institute(CRI), Kasauli, Himachal Pradesh, National Centre for Disease Control(NCDC), Delhi, National Institute of Biologicals(NIB), Noida, and AIIMS, Raipur in various scientific positions.
He has immensely contributed in the field of infectious diseases of bacterial and viral etiologies. He is a nodal officer for any viral outbreak investigation in Chhattisgarh. He has to his credit of detecting first case of H1N1 in India in 2009. He has also investigated at molecular level various outbreaks  viz., Hepatitis B outbreak in Modasa, Gujarat in 2009 , Meningitis outbreak of Delhi in 2008, HEV outbreak in Chhattisgarh in 2014.His area of interest is various diagnostic molecular markers/genes, drug resistance genes, molecular epidemiology and studying the pathogenesis at genetic level in Tuberculosis, Human papillomavirus, HBV and HCV. He has published 25 research papers in national and international journals listed in PUBMED. He has also presented 7 research papers in International Microbiology conferences. He has also been awarded with CSIR young scientist award.
He has also been working as Principal Investigator in Government approved two research project with first entitled asScreening for high risk human papillomavirus (HPV) genotypes and their mutants by real time PCR in women with premalignant and malignant cervical pathology: a hospital based study from Raipur, Chhattisgarh and second entitled as “Molecular profiling of multidrug resistant isolates of Mycobacterium tuberculosis in Raipur, Chhattisgarh.
With a vision of working on developing rapid means of molecular detection of Multidrug Resistant Tuberculosis based on High resolution melt curve analysis to help the clinician in getting the early report of sensitive or resistance pattern of antitubercular antimicrobial for early and rapid management of the MDR TB patient and to reduce further possibility of transmission of bacteria, he would like to join hands with all eminent scientist and researcher working in MDR  tuberculosis.