Seyed Mahmoud Sadjjadi_CVM 2018 - Engii
Biography
Prof. Seyed Mahmoud Sadjjadi
Prof. Seyed Mahmoud Sadjjadi
Shiraz University of Medical Sciences, Iran
Title: Morphologic, morphometric and molecular characterization of Anisakids obtained from edible fish in Bushehr region, Iran
Abstract: 
Background:Anisakid nematodes belong to a number of ascaroid species that normally infect the stomach and intestines of various marine fishes, birds, and fish-eating mammals.
Objectives: A study was made in order to investigate morphological and molecular characterization of ascaroid nematodes obtained from ten edible fishes in the Bushehr region, Persian Gulf, Iran. 
Methods: A total of 519 fish belonging to 10 different species were captured from April to Sept 2016. The ascaroid larvae were detected from fishes by dissection. Different technique including microscopic, molecular, pepsin assay and histopathology were applied for characterization of different species and strains of ascaroid L3 larvae.  
Results: A total of 2119 Ascaroid larvae, including 1793 (%85) Hysterothylaciumspp, 105 (%5) Anisakis type I, 11 (%0.5) Anisakis type II and 210 (%10) as another member of the ascaroid family were detected. The greatest prevalence and intensity of ascaroid infection were recorded for Suridatambil with %42.85 and the intensity up to 55 for Scomberomoruscommerson respectively. There was a significant correlation between prevalence of larvae infection and fish length (P <0.0001). Sequencing of the ribosomal internal transcribed spacer (ITS) regions, the mitochondrial cytochrome C oxidase subuinit II (Cox2) and Restriction Fragment Length Polymorphism (RFLP) using Hinf1 and HhaI enzymes revealed Hysterothylaciumspp as the most dominant isolated nematode. The phylogenetic tree was constructed using appropriate gene data of ITS and COX2 genes. Presence of Aanisakis type I and Hysterthylaciumspp in Suridatambil and Psettodeserumei filets was also confirmed by pepsin assay digestion and histopathology. 
Conclusion: The presence of Hysterothylaciumspp, Anisakis type I and Anisakis type II was confirmed in the edible fishes in the Persian Gulf region which are a potential for human-infecting in this region.

Objective:A study was made in order to investigate morphological and molecular characterization of ascaroid nematodes obtained from ten edible fish in Bushehr region, Iran. 
Methods: A total of 519 fish belonging to 10 different species were captured from April to Sept 2016. The ascaroid larva were detected from fishes by dissection. Different technique including microscopic, molecular, pepsin assay and histopathology were applied for characterization of different species and strains of ascaroid L3 larvae.  
Results: A total of 2119 Ascaroid larvae, including 1793 (%85) Hysterothylaciumspp, 105 (%5) Anisakis type I, 11 (%0.5) Anisakis type II and 210 (%10) as other member of ascoroid family were detected. The greatest prevalence and intensity of ascaroid infection were recorded for Suridatambil with %42.85 and the intensity up to 55 for Scomberomoruscommerson respectively. There was a significant correlation between prevalence of larvae infection and fish length (P <0.0001).Sequencing of the ribosomal internal transcribed spacer (ITS) regions, the mitochondrial cytochrome C oxidase subuinit II (Cox2) and Restriction Fragment Length Polymorphism (RFLP) using Hinf1 and HhaI enzymesrevealed Hysterothylaciumspp as the most dominant isolated nematode. Phylogenetic tree was constructed using appropriate gene data of ITS and COX2 genes. Presence of AanisakistypeIand Hysterthylaciumspp in Suridatambil[HASOUN] and Psettodeserumei [KAFSHAK] filets was also confirmed by pepsin assay digestion and histopathology. 
Conclusion: The presence of Hysterothylaciumspp, Anisakistype I and Anisakis type II was confirmed in the edible fishes in the Persian Gulf region which are a potential for human infecting in this region.

Biography: 
Seyed Mahmoud Sadjjadi is a Professor of Parasitology at the Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Iran. His area of expertise is parasitic helminthes especially zoonotic parasites. The diagnosis and treatment of the parasitic helminthes is his main area of research. He authored or co-authored over 100 scientific papers published in reviewed journals and presented more than 120 research works at international and national conferences. He has co-authored 4 books and wrote a chapter entitled: Epidemiology of echinococcosis in the Middle East and Arabic North Africa In: Advances in Hydatid Disease Diagnosis and Treatment, published on 2013. He had the opportunity to be a research fellow at Liverpool School of Tropical Medicine, Liverpool University and he is member of editorial board of different journals including Iranian Journal of Parasitology, Iranian Journal of Veterinary Research and, International Journal of Enteric Pathogens. He has supervised more than 25 Ph.D. and MSc. theses.