Prof. Hweiyan Tsai
Prof. Hweiyan Tsai
Chung Shan Medical University, Chinese Taipei
Title: The biochemical applications of functional magnetic nanoparticles with homemade magnetic microplates
We would report the applications of bio-labeled magnetic nanoparticles for biomarker detections in the first part of this speech. The efficiency of the immunoreaction was improved by integrating the sandwich immunoassay using functional magnetic and fluorescent nanoparticles with a homemade magnetic microplate. This method has many potential advantages, such as (1) the amount of proteins immobilized on the particles is consistent in the same batch. (2) The functional magnetic particles (MPs) can be made antibody labeling easily. (3) MPs can be dispersed in a solution to yield a pseudohomogeneous reaction with antigens. (4) They can be easily separated and redispersed in the solution by selective using the magnetic force. (5) It is easily adapted with current instruments. The second part of this speech will include the applications of tyrosinase-labeled magnetic nanoparticles on quick screening of true tyrosinase inhibitors from natural products, and efficient determination of ʟ-dopa in complex formulations. Tyrosinase is responsible for the undesired enzymatic browning of fruits that occurs during senescence or following damage incurred at post-harvest handling and processing. These phenomena have led to the search for new potent tyrosinase inhibitors for use in foods and cosmetics. Here, we used a microplate assay integrating tyrosinase-immobilized magnetic nanoparticles (TYR-MNPs) and a homemade magnetic microplate for the high-throughput screening of natural products. This method can screen compounds that actually interact with the active sites of the enzyme, distinguishing them from antioxidants or tyrosinase substrates. This system can also be used for a high throughput and selective determination of ʟ-dopa (levodopa) in complex pharmaceutical formulations.
Hweiyan Tsai is a Prof. of Medical Applied Chemistry at Chung Shan Medical University, Taiwan. She received her PhD from University of Pittsburgh and worked as a postdoc research fellow at biomedical engineering department of Cleveland Clinic Foundation in USA and a project manager of Pharmaceutical Technology Development Programs at Industrial Technology Research Institute in Taiwan. Her major fields of research interest include electrochemical biosensors, magnetic functional nanoparticles for biochemical analyses and screening of functional foods.