Prof. Bechan Sharma
Prof. Bechan Sharma
University of Allahabad, India
Title: The structure and catalytic properties of HIV-1 reverse transcriptase with special reference to drug resistance
Human immunodeficiency virus type 1 reverse trancriptaseHIV-1 reverse transcriptase (HIV-1 RT) is heterodimeric (p66/p51) protein in nature. HIV-1 RT exhibits a half open right hand conformation and hence it has thumb, fingers, palm and connecting domains. The single enzyme possess five different catalytic properties such as RNA dependent DNA polymerase acticity, DNA depenedent DNA polymerase activity, Pyrophosphorolysis activity, RNAse H activity and Starnd transfer activity. The unique properties of human immunodeficiency virus type 1 reverse trancriptaseHIV-1 reverse transcriptase (HIV-1 RT) include its high propensity for misinsertion and misincorporation of deoxyribonucleotide triphosphate (dNTP) in the groing chain’s 3’ terminus during proviral cDNA synthesis. The variations of HIV-1 RT differ in their activities such as extent of formation of binary and ternary complexes as well as in misinsertion and mispair extension of nucleotides in the growing chain of DNA. HIV-1 RT has been shown to evolve strategies to generate drug resistance. The association of the structure-function relatiosnhip as ell as mechanisms of drug resitance would be the light of the three dimensional cryatal structure of the enzyme.
Prof. B. Sharma, currently working as a Professor and HoD, Department of Biochemistry of University of Allahabad, Allahabad has completed his PhD from BHU-Varanasi and Central Drug Research Institute Lucknow India. He has received his Post Doctoral training from UMDNJ-USA for three yers and then served in various capacities i different Universities in India. He has acted as a Visiting Scientist in different Universities/Institutions at USA, Germany, France, Italy, Thailand, Brazil, Iran and Kuwait. He has produced 12 PhD students and guided 6 Post Doctoral Research Fellows. He has published 4 books and about 150 research papers as well as one US patent.