个人简介
Prof. Guillaume TRESSET
Prof. Guillaume TRESSET
Université Paris-Sud, France
标题: Understanding virus self-assembly from X-ray and neutron scattering
摘要: 
Viruses are ubiquitous pathogens in all kingdoms of life and are major public health issues. Despite a huge body of work dedicated to the molecular biology of viral life cycles, there are currently no physical models accounting for the mechanisms by which the hundreds of molecular building blocks making up a virus fit into the final structure with a pinpoint accuracy. I will present first the assembly and disassembly pathways of empty icosahedral capsids. Kinetic models inferred from time-resolved small-angle X-ray scattering (TR-SAXS) data collected with a synchrotron source revealed cooperative mechanisms involving unexpected long-lived intermediate species. I will give some insights into the packaging of synthetic polyelectrolytes studied by neutron scattering (SANS) at equilibrium and demonstrate a nonspecific electrostatic selectivity that may play a crucial role for genome packaging in host cells. The nonequilibrium dynamics of genome packaging was investigated as well by TR-SAXS and I will show that both cooperative and nucleation-growth pathways can be followed depending on ionic conditions. Quite generally, physics provides a useful framework to describe virus self-assembly and should eventually support the development of novel therapeutic strategies.
简介: 
Guillaume Tresset received his master in electrical engineering from the Ecole Supérieure d'Electricité (Supélec) and obtained his PhD in physics at the French Atomic Energy Agency (CEA) in France. He worked at the University of Tokyo in Japan on microfluidics for the manipulation of lipid vesicles and molecular motors, then at the Institute of Bioengineering and Nanotechnology in Singapore on artificial viruses. He is now a research associate with the French National Center for Scientific Research (CNRS) and he works at the Université Paris-Saclay in France. He is an expert in the physics of self- organizing biological matter, notably by using X-ray and neutron scattering, and he develops microfluidic devices for controlling the assembly kinetics of soft nanoparticles. He is an author of more than 90 peer-reviewed journal articles and conference proceedings, and he filed 2 patents for biotechnological applications.