KeyNote Speaker Committee
  • Dr. Gábor Kocsy
  • Dr. Gábor Kocsy
  • Hungarian Academy of Sciences, Hungary
  • Title: Study of hydrogen peroxide-responsive miRNAs and their target genes in wheat
  • Abstract: Adverse environmental conditions results in several yield loss of crops which endangers food safety. A common consequence of the various stresses is the induction of changes in the redox state of plants which can activate several defense mechanisms. Hydrogen peroxide has a central role in this process. Its proposed effect on miRNAs which are post-transcriptional regulator of gene expression was investigated in wheat. Hydroponically grown 12-day old wheat (Triticumaestivum L. cv Chinese Spring) seedlings were treated with H2O2 (10mM) for 0, 1, 3, 6, 9, 12 and 24 h. The endogenous H2O2 content of the leaves was not affected but the amount and redox state of glutathione degrading H2O2, changed. Both the amount of glutathione and glutathione disulphidedecreasedwhile the reduction potential of this redox couple increased. This indicates that the redox environment in the leaves became more oxidizing. Consequently, the expression of the redox-responsive marker genes oxidative stress2, and stromal ascorbate peroxidase1 increased 5-8-fold.Among the 738 miRNAs detected by next generation sequencing 47 miRNAs showed response to H2Oout of which 38miRNAs were up-regulated while only 9 miRNAs were down-regulated. 90 target genes of H2O2-responsive miRNAs were determined bydegradome analysis. Among them the H2O2-induced expression changes were confirmed by qRT-PCR for genes encoding an auxin-responsive protein, an RNA-binding protein, an ATP-dependent protein, a beta-carotene isomerase, a glutathione S-transferase, a ubiquitin hydrolase and a meiosis-related protein. The H2O2 response of selected miRNAs will be checked by Northern analysis and their effect on proposed target genes will be checked by co-expression of miRNAs and their targets in tobacco leaves using Agrobacterium-mediated transformation.If the regulatory interactions can be confirmed, the modifications of miRNA levels by herbicides or safeners affecting the redox state may be used for the improvement of stress tolerance of crops.The project is supported by the Chinese and Hungarian Government, financed by the Research and Technology Innovation Fund (ID: TéT_12_CN-1-2012-0002), by the EuropeanUnion (FP7-KBBE-2011-5, 289842 – ADAPTAWHEAT) and by the Hungarian Scientific Research Fund (OTKA K83642).
  • Biography: Gábor Kocsy was born in Devecser, Hungary in 1964. He is married, and father of 3 children. He obtained his M.Sc. in biology at the Loránd E?tv?s University of Sciences, Faculty of Natural Sciences in Budapest, Hungary in 1989. The topic of the M.Sc. thesis was the study of the effect of osmotic stress in wheat tissue culture. He prepared his PhD in plant physiology between 1992-1995 at the University of Berne, Institute of Plant Physiology in Switzerland. He studied the role of the antioxidants in the response of maize to chilling. He obtained the degree doctor of the Hungarian Academy of Sciences in 2009. In his thesis for this degree he investigated the abiotic stress response in cereals using physiological, biochemical and molecular biological approaches. He is working at the Agricultural Institute of the Centre for Agricultural Research of Hungarian Academy of Sciences, Department of Plant Molecular Biology, Martonvásár, Hungary, since 1989 where he is a scientific consultant since 1989. His research field is the study of the redox control of development and stress response in plants by physiological, biochemical and molecular biological methods. He has the following scientific parameters: cumulative IF – 72.45, independent citations – 790, H-index – 18. He received the following professional prizes: 2nd prize at the National Conference of Students in 1988; 2nd prize of the Veszprem Regional Committee of the Hungarian Academy of Sciences in 1991; prize of the Hungarian Academy of Sciences for Young Scientists in 1998; 1st prize of the Frank-Helianthus Fund in 2002. He participated in the practical education of the students at the Institute of Plant Physiology of the University of Berne (1992-1995) and at the Agricultural Research Institute of the Hungarian Academy of Sciences (1995-). He gives seminars in stress physiology at the University of Veszprém (1998-). He was the supervisor of 3 BSc-, 9 MSc and 2 PhD-students since 2001. He received a Ph.D.-scholarship at the University of Berne, Switzerland (C. Brunold, 1992-1995), a COST-scholarship at the University of Berne, Switzerland (C. Brunold, 1995, 1 month), a DAAD-scholarship at the Goethe University, Frankfurt am Main, Germany (L. Nover, 1998, 3 months), a Bolyai János Research Fellowship (1998-2001), a TéT-fellowship at ARC Roodeplaat, South Africa (J. A. de Ronde, 2000 an 2001, each 1 month), an E?tv?s scholarship at the University of Tucson, USA (J.-K. Zhu, 2005, 3 months) and a fellowship from the Plant Resource Program, IPK, Gatersleben, Germany (N. Stein, 2005, 10 months). He was the president of the Biothechnological and Plant Physiological Working Group of the Veszprém Committee of Hung. Acad. Sciences between 2000 and 2011. He is member of the Society of Hungarian Plant Biologists, the Federation of European Societies of Plant Biologists and the Society of Hungarian Entomologists. He was principal investigator in 6 national scientific grants and in 6 bilateral international research projects. He is editor in the “Advances in Agriculture” and “The Scientific World Journal”. He is regularly reviewer of national and international research grants and scientific publications.
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April 14-16, 2015
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December 11, 2014  >> February 16, 2015
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