Nuclear Magnetic Resonance (NMR) is an evolving technology which plays important roles in the biological and biomedical research, and either the spectrometers or methods of NMR has developed a lot recently. National Center for Protein Science ¡¤ Shanghai (NCPSS) officially launched in Dec. 2013, and NMR is one of the principal technologies in the NCPSS. The NMR facilities includes Bruker 900 and 600MHz NMR spectrometers, Agilent 800, 700 and 600 MHz NMR spectrometers. Except for the Agilent 700MHz spectrometer equipped with a room temperature probe for liquid state NMR and a Bio-MAS solid biological probe, all the other spectrometers are equipped with both room temperature probes and cryogenic probes for solution NMR respectively. As the NCPSS passing the national acceptance in May. 2015, the NMR facility have the ability to support a lot of NMR application, including folding optimization of protein samples, study protein solution structure and function relationship at atomic resolution, and investigation on the dynamics and interactions of proteins.
The NMR facility is committed to serve for the scientific researches especially in the study of the structure and function for bio-macromolecules. And the facility has a high-level technical team which can provide comprehensive NMR technology support for those structural biologists who are unfamiliar with NMR technology. This support includes NMR data collection, spectrum alignment and structural calculation. Meanwhile, with the help of Prof. James J. Chou and other experts, we have launched on the study and develop on some state-of-art technology of Bio-NMR based on our instruments. Combined with the various experimental requirements of different users, several advanced Bio-NMR technology systems has been set up and published, including 1) general triple-resonance experiments on small protein and methods to solve solution structures based on Nuclear Overhauser Enhancement (NOE), 2) high-sensitivity TROSY-type triple-resonance experiments for larger membrane proteins, 3) several methods for Non-uniform Sampling (NUS) data collection and spectrum reconstruction, 4) TROSY-CPMG relaxation dispersion experiments for dynamic studies, 5) NMR data collection for RNA structure and function, 6) NMRlego software which based on Residue Dipolar Couplings(RDCs) for the molecular fragment replacement of membrane proteins (new method development).
In the past three years, NMR facility has served 13 organizations all around the world for about 47 projects, including 2 Noble laureates¡¯ research groups. The users published achievements involve many fields, such as the structures and functional study of membrane proteins, protein dynamics, weak protein-ligand interactions, NMR methodology and so on. Some references are shown in the page of this Chinese abstract.
Both academic and industry users are welcome to apply the NMR facilities for bio-macromolecular experimental research and collaborate with us.Biography:
1999-2003 B.S. in Physics Inner Mongolia University, Inner Mongolia, China
2004-2009 Ph.D. in Structure Biology University of Science and Technology of China, Hefei Advisor: Prof. Yunyu Shi
2009-2012 Postdoctoral Study High Magnetic Field Laboratory of CAS, Hefei (In Biomolecular NMR ) Advisor: Prof. Junfeng Wang
2012-now Associate Professor National Center for Protein Science Shanghai, Institute of Biochemistry and Cell Biology, (NMR Facility Manager) Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences (CAS), Shanghai Advisor: Prof. James J Chou
The long-term interest of my research is in understanding the molecular structures and dynamics of Bio-macromolecular by solution NMR spectroscopy. Although we have five high magnetic field NMR instruments, such as Bruker 900MHz and Agilent 800MHz spectroscopy, for applying them to address important questions in biology. Our major challenge is to push the technological envelope of solution NMR to larger and more complex Bio-macromolecular systems. Combined with the various experimental requirements of different users, several advanced Bio-NMR technology systems have been set up and published, including, 1) high-sensitivity TROSY-type triple-resonance experiments for larger membrane proteins, 2) several Non-uniform Sampling (NUS) data collection and spectrum reconstruction methods, 3) TROSY-CPMG relaxation dispersion experiments for dynamic studies, 4) NMR data collection for RNA structure and function, 5) NMRlego software which based on Residue Dipolar Couplings(RDCs) for the molecular fragment replacement of membrane proteins.