Dr. Evgeny Puchkov
Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russia
Image analysis in microbiology
This presentation is a review focused on using computer image analysis as a means of objective and quantitative characterizing optical images of the macroscopic (e.g. microbial colonies) and the microscopic (e.g. single cell) objects in the microbiological research. This is the way of making many visual inspection assays more objective and less time and labor consuming. Also, it can provide new visually inaccessible information on relation between some optical parameters and various biological features of the microbial cultures. Of special interest is application of image analysis in fluorescence microscopy as it opens new ways of using fluorescence based methodology for single microbial cell studies. Examples of using image analysis in the studies of both the macroscopic and the microscopic microbiological objects are presented and discussed.
Dr. Evgeny Puchkov currently holds the positions of Leading Researcher at the Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences (Pushchino, Moscow Region) and Head of the Microbial Preservation Group at All-Russian Collection of Microorganisms which is a department at the Institute (1990-present). Also, he is Lecturing Professor at the State Institute of Natural Sciences (Pushchino, Moscow Region) for Master Degree students (1993-present). Previously, he was a Research Fellow, Senior Research Fellow and Head of Laboratory at the Institute of Applied Microbiology (Obolensk, Moscow Region) (1976-1990). He received a Master and a Candidate of Sciences (equivalent to PhD) degrees in Biophysics from the 2-Medical Institute (now University) of Moscow (1972 and 1976, respectively) and a Doctor of Sciences degree (the highest Scientific Degree in Russia) from the Institute of Applied Microbiology (1989). He is expert in various spectroscopic, microscopic and microbiological methods including long-term maintenance of live microbial cultures. His main scientific achievements are associated with the development of new approaches for both fundamental and applied research in microbiology. The methods he developed were successfully used for investigating intracellular physical-chemical parameters, functional features of biological membranes, cell viability assessment, mechanisms of damaging effects of low temperature and some antimicrobials on microorganisms, to mention a few. For the last decade, he is most interested in combining computer image analysis with optical methods for retrieving new information from microbiological objects. He has published more than 150 papers in journals (including several popular science papers), a book chapter, Author Certificates of Inventions and conference proceedings both in Russian and in English. He serves as a reviewer in international journals World Journal of Microbiology and Biotechnology, Acta Nature, Biochemistry (Moscow) Supplement Series A: Membrane and Cell Biology.
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